IMAGING IMMUNITY – from Nanoscale to Macroscale | Insights from Biophysics
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Invited Lecture | Diane Lidke, Albuquerque

Session chair: Gilbert Fruhwirth (London, UK)
Date: Wednesday, 15 January, 2020, 8:45 AM - 9:30 AM


8:45 AM -01

Dissecting Signal Transduction, One Molecule at a Time: Using Single Molecule Imaging to Understand FcεRI Signaling Mechanisms (#46)

Diane Lidke1

1 University of New Mexico, Pathology, Albuquerque, New Mexico, United States of America


The recruitment of intracellular signaling molecules to membrane receptors is a critical step in signal transduction.  In mast cells, Syk binding to the phosphorylated ITAM is the key step in propagating FcεRI signal transduction to functional outcomes. In fact, loss of Syk expression in mast cells completely abrogates the release of granular contents or cytokine production after FcεRI activation. Yet, the molecular mechanisms controlling Syk-ITAM binding are not completely understood, partly due to a lack of methods for monitoring individual protein behavior in living cells. Fluorescence microscopes are now becoming fluorescence “nanoscopes” as single molecule and super-resolution imaging go beyond the diffraction limit. These high-resolution techniques are enabling the quantification of protein dynamics and distribution in live cells. We have used single molecule imaging to map the dynamic interactions of FcεRI with downstream signaling partners, including Lyn, Syk and SHIP-1. This approach provides new fundamental insight into how signaling molecules interact with, and compete for, ITAM binding sites.



Keywords: fluorescence microscopy, single molecule imaging, Biophysics, cell signaling